goat anti human tf polyclonal antibody  (Bethyl)

Journal: TH Open: Companion Journal to Thrombosis and Haemostasis

Article Title: Sensitive and Specific Detection of Platelet-Derived and Tissue Factor–Positive Extracellular Vesicles in Plasma Using Solid-Phase Proximity Ligation Assay

doi: 10.1055/s-0038-1667204

Figure Lengend Snippet: Detection of TF+ EVs using SP-PLA and CT-B capture. ( A ) The levels of TF+ EVs was measured with SP-PLA by using CT-B capture in unstimulated plasma or in plasma from TRAP or LPS-stimulated whole blood with or without triton-X100 treatment. Levels relative to EV-free plasma are shown. ( B ) The levels of TF+ EVs were measured with SP-PLA in plasma samples from unstimulated and stimulated whole blood from two individuals before (white bars) and after (black bars) centrifugation at 20.000 × g for 60 minutes. Levels relative to buffer alone are shown. Error bars represent standard deviation from technical replicates ( n = 2). ( C ) Levels of TF+ EVs were measured by SP-PLA using the TF polyclonal goat antibody conjugated to both PLA probes as usual (black bars) or by using the TF polyclonal goat IgG (conjugated to probe 1) together with a control goat IgG (conjugated to probe 2) (gray bars). The levels relative to signal in buffer alone is shown. ( D ) Levels of TF+ EVs were measured with SP-PLA using CT-B capture in plasma from LPS-stimulated whole blood, diluted 1:2 in a dilution series. The plasma concentration is plotted against linearized CT values. Error bars represent standard deviation of technical replicates ( n = 3).

Article Snippet: Goat anti-TF polyclonal antibody (AF2339) and goat IgG (AB-108-C) were from R&D Systems (Minneapolis, Minnesota, United States).

Techniques: Clinical Proteomics, Centrifugation, Standard Deviation, Control, Concentration Assay